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Biology at Washington & Jefferson College

Technology Manual

Autoclave instructions

Balance, electronic (Room 011)

Balance, electronic (Room 102)

Banner program

Camera in cell culture room

Capturing pictures using the video camera

CD burner

CD-ROM's and software shares

Copy machine, Toshiba

Digital camera

Kodak Edas 290 for photographing gels

Laminar flow biological safety cabinets

Laptop briefcase checklist

Laptop briefcase operation

pH meter (Room 011)

pH meter (Room 102)

Scanners

Sony video camera-microscope projection system

Thermocyclers, Perkin Elmer

TV/VCR

Video projector, LED (Panasonic and Proxima)

Video projector, EPSON PowerLite 735c Multimedia Projectors

Water purification system, Nanopure

Yost Smart Classroom steps

 

Autoclave Instructions

ROOM 211B

*MAKE SURE TO SIGN IN BEFORE USING- SIGN IN SHEET IS ON THE WALL TO THE LEFT*

  1. Check that the yellow water valves - behind the machine near the floor - are open. They are open when they are at a 45o angle to the pipes.
  2. The Eagle emblem on the touchpad means that the unit is in the Standby mode.
  3. Touch the Eagle. If it is the first use of the day the "Generator Flush" pad will appear. Follow the instructions as stated. [If the sterilizer has been used earlier that day the "Generator Flush" will be bypassed.]
  4. Check the jacket pressure gauge behind the front door (not chamber pressure). It must read about 10 psi. To open the front door (not the chamber), pull on the left side of the door.
  5. Open door of chamber manually or by stepping on automatic lever on the bottom right.
  6. Load chamber. CAUTION: If you are sterilizing liquids, you must use a container that will hold twice the volume that you wish to autoclave (2L flask for 1L of liquid). You must also place your flasks, etc. in an autoclavable pan- these are the large white pans under the bench to the right of the autoclave.
  7. Close door manually or by stepping on the lever.
  8. The main menu now appears. You are asked to select a cycle. Use a liquid setting for liquids and gravity for non-liquids. If you press one of the cycles, the parameters will appear- if this is not what you want to do, press previous to get back. Otherwise, press start cycle. See the manual on the inside front cover for more guidelines.
  9. Select the cycle. It should take roughly 50-55 minutes. When completed, a signal goes off.
  10. Open the door to the chamber. If there has been a spill, contact Dr. Dryden immediately, and make sure that the autoclave is not re-used until the spill is cleaned up (attach a sign to the autoclave so that others know not to use it).
  11. Remove the sterilized materials- make sure you wear autoclave gloves. If you sterilized liquids, gently shake the flask before removing it as sometimes liquids superheat and boil up when agitated. If the liquid does boil up, leave it for several minutes before removing.
  12. Press on "options" on the touchpad. Then press "Next" on the following touchpad. Finally, press on "Standby" and the autoclave is ready and holding for the next run.

QUESTIONS/PROBLEMS: SEE DR. DRYDEN.

 

Balance, electronic

ROOM 011

*THIS BALANCE WEIGHS ITEMS BETWEEN 0.05 GRAMS (50mg) AND 200 GRAMS*

  1. Brush off balance using the brush beside it if necessary.
  2. Make sure balance is plugged in.
  3. Press "on/tare" button.
  4. Make sure balance is zeroed. Press "on/tare" if it does not read 0.00g.
  5. Place  a weighboat on the scale- these are available in the drawer under the balance; larger ones are available in the cabinet below the drawer (cabinets are labeled).
  6. Tare weighboat.
  7. Place object on weighboat and take reading.
  8. Press "off/mode" when finished.
  9. Brush off balance using the brush beside it after use and clean any spills.
  10. Replace balance in drawer. 

QUESTIONS/PROBLEMS: SEE DR. McGRAIN.

 

Balance, electronic

Room 102

*THIS BALANCE WEIGHS ITEMS BETWEEN 0.05 GRAMS (50mg) AND 4000 GRAMS*

  1. Brush or wipe off balance before use.
  2. Press "on/tare" button.
  3. Make sure balance is zeroed. Press "on/tare" if it does not read 000.00.
  4. Place object or sample to be weighed on scale. Samples should be placed in a weigh boat or on weigh paper.
  5. Tare weighboat between measurements.
  6. Press "off" when finished.
  7. Brush or wipe off balance after use.

QUESTIONS/PROBLEMS: SEE DR. LEE.

 

Banner Program

Room 103

  1. Open application by double-clicking on PrintShop icon on desktop.
  2. Click on "banner."
  3. Follow the instructions for production of banner.  Typically, use landscape format.  For a plain text banner, choose "start from scratch."
  4. To change text size, highlight text, click on "Text" on tool bar and then "Font."  This will give you a wider range of font sizes that those available on the tool bar.
  5. Print banner.  Unlike previous versions of PrintShop, this one doesn't seem to print page cutting guidelines.

QUESTIONS/PROBLEMS:  SEE DR. McGRAIN

 

Camera in Darkroom

Room 002A

  1. Place gel down with plastic wrap.
  2. Make sure timer is on (not on hold).
  3. Turn on visible light.
  4. Make sure red filter is not in place, took through lens and focus with the knob on the left.
  5. Set aperture on F4 and time = 1/8 sec (not F8, 1 sec as previous instructions).
  6. Put red filter in place.
  7. Make sure goggles are on.
  8. Move film packet to left.
  9. Turn off all visible light.
  10. Turn on transmitted UV light.
  11. Use trigger to take picture.
  12. Move film packet to right.
  13. Pull out picture by white strip.
  14. Turn UV light off.
  15. Turn visible light on.
  16. Turn timer off.
  17. Wait 2 minutes and peel off picture.

QUESTIONS/PROBLEMS: SEE DR. DeBERRY

 

Capturing Pictures Using the Video Camera

  1. Establish that video camera is properly hooked up.
  2. Turn on computer.
  3. Turn on microscope (light). In the case of variable lights turn to full power.
  4. Select Biology Utilities from desktop.
  5. Double Click Weyes.512
  6. If everything is working an image should appear. If a blank background appears adjust slide to find sample.
  7. Once you have an image click on preview.
  8. Click on AutoCAl.

To capture:

Video

  1. Click Freeze, then click Capture.
  2. Open File.
  3. Save As.
  4. Name File.
  5. Click OK.

Slide/Still Frame

  1. Click Capture.
  2. Open File.
  3. Save As.
  4. Name File.

QUESTIONS/PROBLEMS: SEE DR. McGRAIN

 

CD Burner

ROOM 103

  1. Insert Compact Disc into drive
  2. Click on “Burn CD & DVDs with Roxio.Ink”
  3. Choose the type of disc you want to burn (i.e. Audio or Data)

For Data CD you can choose from:

·        Direct CD – drag files to CD using     Windows Explorer

·        DataCD Project – archive computer files in most compatible form

·        DataDVD Project – used to store up to 4.7 GB of data

  1. Follow instructions on screen

QUESTIONS/PROBLEMS:  SEE DR. McGRAIN

 

CD-ROM's and Software Shares

*THIS CAN BE DONE FROM ANY COMPUTER IN THE COLLEGE, AS LONG AS THE COMPUTER IS NETWORKED*

  1. Right Click on My Computer
  2. Select “Map Network Driive”
  3. Choose an Open Drive
  4. Type:  //washjeff.edu/shares/biology
  5. Click Finish

QUESTIONS/PROBLEMS: SEE DR. McGRAIN
 

Copy machine, Toshiba

Room 103

*THE ON/OFF SWITCH IS ON THE RIGHT SIDE OF THE MACHINE, VERY CLOSE TO THE FRONT.  THE MACHINE SHOULD NOT BE TURNED OFF DURING THE DAY, AS IT TAKES A WHILE TO WARM UP.  INSTEAD, PRESS THE “ENERGY SAVER” BUTTON WHEN THE MACHINE IS NOT IN USE.

THE MANUAL FOR THE COPY MACHINE IS IN A TRAY AT THE BACK OF THE MACHINE CONSULT THIS IF YOU HAVE ANY QUESTIONS.*

To make one-sided copies from a one-sided original

  1. Unless originals are wrinkled, stapled, excessively folded, or wet, you can use the document feeder tray instead of opening the top of the machine and placing the original on the glass.
  2. Put the originals printed side up in the document feeder tray and press the green "print" button.
  3. If you want more than one copy, enter the number you would like on the numeric keypad. You can correct errors by pressing the orange "clear" button. NOTE: This machine does not collate or staple- this must be done manually.
  4. If you need to enlarge or shrink the original, use the 200%, 100%, and 50% buttons on the upper left hand side.
  5. If the copies are too dark or light, change this with the "light dark auto" buttons on the lower left hand side.
  6. If the original contains a picture, pressing the "image mode" button will improve the quality of the copies.

Duplexing

  1. This term applies to copies that are anything other than one-sided copies made from a one-sided original (1:1). NOTE: You can only duplex 50 copies at a time.
  2. To make double-sided copies from single-sided originals, select the 1® 2 button from the duplex list (far left) by pressing the duplex button once. A green light will come on beside the 1® 2 picture.
  3. Place the originals in the document tray and follow the on-screen instructions. You are asked the following questions; respond yes or no by pressing the appropriate button:
  4. Want original count? (NO- unless you do)
  5. Odd # of originals? (YES, if you have 3, 5, etc. originals- otherwise your first page will be one-sided; NO if you have an even number of originals)
  6. Want help with duplex copying? (probably NO)
  7. Press print
  8. This same procedure works if you want double-sided copies from double-sided originals (2:2) or single-sided copies from double-sided originals (2:1). Make sure that you press the duplex button to turn on the green light beside your selection (you will have to press it more than once).
  9. The copy machine will also make copies from books- make this selection from the duplex menu (book® 2) and place the book on the glass. This can be very convenient- you will not have to continuously move the book around to copy the pages.

Troubleshooting

  1. If anything goes wrong, indicated by the copy machine stopping, there will be instructions on the screen telling you how to fix it. Do not leave if something goes wrong! If you must, leave a note on the machine with details of the problem.
  2. Hint: If you are instructed to open the front cover and press lever A to open the top of the machine, you will need to lean down slightly on the back left side of the copier. If you do not do this, the top will not open.
  3. If you have to replace the toner, on-screen instructions will appear. The toner is kept in a box on top of the mailboxes. Do NOT throw out the old toner container, as these are recycled. Simply place the old container in the marked box.

QUESTIONS/PROBLEMS: SEE DR. McGRAIN

 

Digital Camera

Stored in Room 205

  1. Remove the lens cap.
  2. Turn the mode dial to the "A in a box and camera" picture.
  3. Turn the camera on.
  4. Zoom in using the “T” button and out with the “W” button.
  5. Focus by depressing the shutter release halfway.
  6. Take the picture by fully depressing the shutter release.
  7. To review the pictures press the QUICK arrow button.
  8. When finished turn the camera off to save the battery.

Connecting to a computer

  1. Connect using the USB cable.
  2. Turn on the camera.
  3. Then push the transfer button on the camera.

Connecting to a television or a VCR

  1. Connect the video cable to the camera.
  2. Connect the video cable to the video device.
  3. Tune the television to the video channel.
  4. Turn on the camera..    

 

Kodak Edas 290 for Photographing Gels

Room 211

*MUST BE CLEARED BY DR. DRYDEN TO USE.*

  1. Log onto the server.
  2. Go to Programs in the Start Menu.
  3. Open Kodak ID 3.6 Software.
  4. If you have never used the software before, it may ask you for standard settings, select Hae Standards or whatever molecular weight standard you are working with. (If you need to change the molecular weight standard later, consult the Kodak ID 3.6 user manual on how to change standards.)
  5. If you have never used the software before, it may also be unable to find the camera.  Go to “File” and click “Select Digital Camera.”  In the drop down boxes, you should select “Digital Camera:  DC 290, Port:  USB, Connection  Speed:  Auto.”
  6. Lower the plastic window on the EDAS 290.
  7. If necessary, change the matting to fit the size gel you are using.
  8. Cover the stage with plastic wrap to prevent contaminating the machine.  (Try to make the plastic as smooth as possible.  This will help with the clarity of your photograph.)
  9. Place your gel on the stage and slide the platform door closed.
  10. If you are using EtBr, make sure to turn on the UN light source.  The on/off switch is a green button located on the base of EDAS.
  11. In the top left corner of the Kodad ID program, click on “Capture DC 290.”
  12. Select you sample type (i.e. Ethidium Bromide).
  13. Click “Take Preview” to get a preliminary image of your gel and adjust the rotation and size, if necessary.
  14. Click “Take Picture.”
  15. If you are satisfied with your photograph, click “Close DC 290.”
  16. In the “Image Display” adjust the contrast as necessary.
  17. To print your image, go to “File” and “Print.”
  18. Make sure that you select “HPDJ820.”
  19. Congratulations!  You’ve successfully printed a digital image of your gel.
  20. Turn off the UV light source, open the door to the platform, and remove your gel and plastic wrap.
  21. Wrap your gel in plastic wrap and aluminum foil and label it with your/your group’s information and the date.  Place your gel in the refrigerator.  It is important to wrap your gel in aluminum foil if you are using EtBr, because it will degrade when it is exposed to light.
  22. Save your project in Kodak ID 3.6 to your home folder.

QUESTIONS/PROBLEMS:  SEE DR. DRYDEN

 

Laminar Flow Biological Safety Cabinets

In General:

  1. Minimize penetration of "air curtain"
  2. Minimize room activity
  3. Do not transfer viable materials or manipulations that may generate aerosols above sterile or uninoculated materials. Items should be localized on the work surface in "clean" and "dirty" groups.
  4. Employ aseptic technique

Specifically:

  1. Turn on blower and lights
  2. Allow blower to operate for a minimum of 15 minutes before use.
  3. Wipe down internal surfaces with 70% alcohol (do not use bleach)
  4. Place materials and equipment into the cabinet.
  5. Wait 2-3 minutes.
  6. Perform work, as far into the cabinet as possible.
  7. After completion of work, remove materials and decontaminate cabinet interior with 70% alcohol.
  8. Check and clean paper catch weekly.
  9. Turn off blowers and lights.

QUESTIONS/PROBLEMS: SEE DR. DeBERRY or DR. DRYDEN

 

Laptop Briefcase Checklist

  1. Computer
  2. Instruction manual
  3. Instruction sheet
  4. AC cord and adapter
  5. Modem cable
  6. Ethernet cable
  7. Ethernet adapter
  8. CD-ROM OR floppy drive in separate compartment
  9. FDD cable

QUESTIONS/PROBLEMS: SEE DR. McGRAIN

 

Laptop Briefcase Operation

*THE LAPTOP IS A DELL 3800 INSPIRON AND USES WINDOWS 2000 AS THE OPERATING SYSTEM.  YOU WILL NEED TO LOG ON TO THE LAPTOP JUST AS YOU DO TO YOUR DESKTOP COMPUTER.  HOWEVER, YOU DO NOT ALWAYS NEED TO HAVE THE LAPTOP CONNECTED TO THE CAMPUS NETWORK VIA ETHERNET CABLE*

  1. THE FIRST TIME you log on to the laptop, however, you MUST be connected via campus ethernet. To do so, use any ethernet cable (the one attached to your desktop, or the short one in the laptop case) and the "dongle," which is just an adaptor that attaches to the left side of the laptop, in the only place on the left side where something could be attached. Simply plug the ethernet cable into the other end of the dongle. Once you have attached the dongle to the ethernet cable, you can turn on the computer- NOT BEFORE!
  2. To open the laptop, press the button on the front and raise the screen. To turn the laptop on, press the grey button above the keyboard.
  3. The laptop battery is quite long-lasting (easily 2-3 hours of use) and should be used when possible. If not, the battery "forgets" that it works and then we have no more battery! Therefore, the battery should be ALMOST run down approximately once a week. When using the battery for power, check frequently to see how much charge the battery has (blue Fn key on bottom left, and F3 at same time; to get out of this display, press Esc). If the battery has less than 10% of its charge remaining, either plug it back in or shut down. If you continue working and the laptop runs out of power completely, you will lose information.
  4. Once you have logged on for the first time, you no longer need to be attached to the ethernet cable to use the laptop. There are two options:
  5. Use the laptop without being connected to the network- if you don't connect via ethernet or by dialing in to W&J (explained below), you can use the laptop but not access your files on the H drive. You can save items either to a floppy disk or the C drive of the laptop. If you wish to transfer these items to your H drive later, you can do so. Keep in mind that anything you save to the laptop C drive will be available to anyone else using the laptop! Only items in your H drive are protected.
  6. Dial up networking- to do this, connect your phone cord to the phone port on the right side of the laptop. Turn on the laptop and go through the usual log on procedures. You will see a message telling you that no domain server can be found- click OK. Do not EVER click on the box "use dial up networking" that appears when you are entering your user name and password.
  7. Once you are logged on to the computer, double click on the Dial-up Networking icon on the desktop. The laptop is set up to dial several numbers; use the arrow to select the number you want:
    • 724 number- use if you are off campus but can make a free local call to W&J
    • 888 number- use if you are off campus but cannot dial W&J for free
    • 9-888 number- use if you are at a hotel requiring you to dial 9 for an outside line
    • 8-888 number- use if you are at a hotel requiring you to dial 8 for an outside line

Dial up networking is about 100 times slower than the campus network, so you should expect to wait for things to happen. This is especially true if you are accessing larger files on your H drive. If you know that you will be working on large files, you may want to consider moving these files to the laptop C drive (using Windows Explorer) while still connected to the campus Ethernet network. This will save you a lot of time, and you won’t need to tie up a phone line to work at home. Once you are back on campus, simply move the files back to the H drive and delete them from the C drive.

Connecting to the H drive from off-campus:

  1. Once you have logged on and dialed in, you can retrieve e-mail with Microsoft Outlook Express and surf the web. You have to go through one more step to get to the H drive though.
  2. Right click on my computer, choose map network drive, and change the drive to H; click OK
  3. The laptop has both a CD-ROM drive and a floppy disk drive, which can be exchanged for one another internally. Or the floppy disk drive can be attached externally so that both the CD-ROM drive and the floppy drive can be used simultaneously. If the floppy drive is installed internally, the CD-ROM drive cannot be installed at all. The floppy drive is in the laptop case, as is the cable to attach it to the laptop.

QUESTIONS/PROBLEMS: SEE DR. McGRAIN

 

pH Meter

Room 011

*THIS BRIEF INSTRUCTION SHEET DOES NOT REPLACE BEING TRAINED ON THE INSTRUMENT BY AN EXPERIENCED USER OR STUDY OF THE DETAILED INSTRUCTION MANUAL.*

  1. Check the screen to determine if the desired parameters are displayed:
  2. pH mode at upper left
  3. number of decimal places in measurement
  4. buffer group
  5. temperature units
  6. If your desired parameters are not displayed, use either the mode key (for pH, mV or Rel mV) or the setup key to scroll through all of the different options. When you find each desired parameter, hit enter. Hitting enter always takes you back to the measure screen. To change another parameter, you must scroll through all those that precede it in the sequence.
  7. When the parameters are set:
  8. Press the setup key twice and then the enter key to clear any existing standardization.
  9. Immerse the rinsed electrode into one of the colored standard buffers.
  10. Press std to access the Standardize mode.
  11. Press std again to initiate standardization. The meter will automatically recognize the buffer and flash the value on the screen. When the "stable" icon appears, the meter returns to the Measure screen.
  12. Repeat steps 2-4 with a second and subsequent buffers.
  13. The slope will be briefly displayed, should be 90-100%, which results in the "GOOD ELECTRODE" message appearing. (Otherwise, "ELECTRODE ERROR" message will appear. In this case, do not use the meter, but notify Dr. McGrain immediately.)
  14. If the electrode is good, immerse the electrode into the sample solution and stir moderately.
  15. Wait for "stable" icon to appear and then record the measurement.
  16. Rinse the electrode and store it how you found it: either in storage solution or with a cot on the tip.

QUESTIONS/PROBLEMS: SEE DR. McGRAIN.

 

pH Meter

Room 102

Things to Remember:

  1. Always store the electrode and temperature probe in the parafilm-covered beaker of deionized water. Do not poke the electrode into the parafilm when returning it to the water.
  2. Do not touch the tip of the electrode- skin oils and dirt can clog the pH-sensitive glass membrane. Fingers can transfer static electricity to the electrode, causing faulty measurements.
  3. Do not crunch the tip of the electrode into the bottom of a beaker or the magnetic stir bar. Do not drape unsecured electrodes over the edge of a beaker when using the magnetic stir-plate!
  4. Always rinse the electrode and temperature probe with a stream of deionized water before and after placing it in a solution.
  5. Before you use the pH electrode, move the rubber sleeve to uncover the hole in the pH electrode. Replace the sleeve after use.
  6. Standardize the pH meter before each use session.

Standardizing the pH meter

  1. Select two appropriate pH standards. (Our pH standards are 4.0, 7.0, and 10.0. Pick two standards, which bracket the pH you wish to measure. To measure the solution at 9.4, select the 7.0 and 10.0 standards.) Be sure that the standard beakers have been replenished recently.
  2. Rinse the electrode and probe and blot them with a dry kimwipe. Place the elelctrode and temperature in probe 1.
  3. Take the pH meter out of standby mode and put it into pH mode.
  4. Press the STBY/MEAS button once.
  5. After the display stops changing, be sure that the display reads "TP PROBE pH.". If not, press the MODE button until the display changes to "TP PROBE pH".
  6. Press the TWO POINT CAL key to do a two-point pH calibration.
  7. The display will read ENTER BUFFER 1. Type in the pH of your first buffer, e.g. "7.00", then hit the ENTER button.
  8. The display will read ENTER MV 1. When the readout of numbers (in mV) has stabilized, hit the ENTER button.
  9. The display will read ENTER TEMP 1. Enter the temperature (25.00).
  10. The display will read ENTER BUFFER 2. Rinse the electrode and probe and blot them with a dry kimwipe. Place the electrode and temperature probe in your buffer 2.
  11. Type in the pH of your second buffer: "10.00" and hit the ENTER button.
  12. The display will read ENTER MV 2. When the readout of numbers (in mV) has stabilized (this may take several minutes), hit the ENTER button.
  13. The display will read ENTER TEMP 2. Enter the temperature (25.00).
  14. The efficiency will appear. Hit ENTER. You are now ready to measure pH.
  15. Reading pH
  16. Rinse and blot dry the electrode and probe. Place them in the solution to be measured.
  17. Read the pH.
  18. When finished, rinse and blot dry the electrode and probe. Return them to the deionized water.
  19. Hit the STBY/MEAS button to return the pH meter to standby. Replace electrode/probe in dH2O.

QUESTIONS/PROBLEMS: SEE DR. LEE.

 

Scanner

 Room 103

  1. Double click on “HP PrecisionScan Pro” icon.
  2. Lift the scanner cover, place the material on the scanner and close the cover. Click on “Scan” in the toolbar and highlight “Preview.”
  3. After the scanned image appears, Click on “Output Type” and make sure “True Color” is checked.
  4. If you want to have less of the scanned image than is currently shown (i.e. you just want a small figure out of a whole page of text), you can do this. Click on the Zoom Scan icon in the toolbar. Then move the cursor (now a cross) to the area of the image that you want, and draw a box around your figure by clicking (and holding) at one of the corners and dragging until you have a box around the desired part of the image. Once you are satisfied with your "box," release the mouse button. If you are not happy with this "box," click outside the image area and start again.
  5. Click the Zoom button; the scanner will scan the area in your "box."
  6. If you wish to adjust the size of the image, move the button directly above the width and height measurements- you will need to play with this to get a feel for the ideal size of your image.
  7. Make final adjustments by using the commands in the “Tools” drop down menu.
  8. When you are ready to save, choose “Save As” in the “Scan” menu and save to disk or H drive.
  9. Take document out of scanner.

QUESTIONS/PROBLEMS:  SEE DR. McGRAIN

 

Sony video camera- microscope projection system

THESE FIRST 4 STEPS APPLY TO ALL MONITORS IN THE DEPARTMENT

  1. Obtain the camera, black woven cord, and converter box- ask Dr. McGrain if you are not sure where these items are.
  2. Insert the wide black plug at one end of the black woven cord (marked AMP) into the RGB SYNC port on the camera.
  3. Insert the gray plug at the same end of the black woven cord into the DC IN port on the camera.
  4. Insert the gray cord at the opposite end of the black woven cord into the converter box (you can use either the top or bottom port).

FOR THE BLACK MONITORS ON THE COUNTERS IN THE BIO 101/102 LABS

  1. At the other end of the black woven cord (the end not attached to the camera), you will find three extensions:
  2. Black woven cord extension- plug this into the S VIDEO port.
  3. Gray cord extension- plug this into the converter box (top or bottom port).
  4. Four cords with colored tape- plug these, in any order, into the top row of ports labeled line A and line B.
  5. Note: If these plugs have a 1" attachment in them, the attachments will need to be removed before inserting the plugs into the ports on the monitor.
  6. Plug in and turn on microscope, converter box, and monitor. Make sure to turn on the light on the microscope.
  7. If the image is not perfect, press the control button on the front right hand side of the monitor; other buttons will light up.
  8. If the picture is black and white, press the line A button, then the VTR button.
  9. If the colors are distorted, adjust using the hue, color, sharp, and bright buttons on the front left hand side of the monitor.
  10. If these steps fail to produce a viewable image, check that the microscope is turned on and a slide is in place.
  11. NOTE: The camera will make the microscope top-heavy and prone to tipping.
  12. NOTE: With some microscopes you need to put a blank slide under the slide that you wish to view in order to get the image in focus on the monitor.

FOR ALL MONITORS

  1. You will need to make adjustments in focus when you display a slide on the screen; what you see under the microscope will not be exactly what you see on the screen. You may also have to increase or decrease the light on the microscope.

QUESTIONS/PROBLEMS: SEE DR. McGRAIN

 

Thermocyclers, Perkin Elmer
(GeneAmp PCR System 2400)

*This brief instruction sheet does not replace being trained on the instrument by an experienced user or study of the detailed instruction manual.*

  1. Turn on at least 10 minutes before your intended use. This allows the cooler in the machine to work optimally.
  2. Turn on main power switch on the left side of the instrument.
  3. Be certain that you are using "domed PCR tubes". Flat top tubes will not work in these thermocyclers.
  4. You must insert the PCR tubes in the gray tray and place them together in the heating well area. Then close the lid so that PCR tubes are in contact with the heated lid.
  5. Press F1-Run from the Main menu.
  6. Use the up and down arrow keys to select the method you want to run.
  7. Press F1-Start. This displays the Reaction Volume screen.
  8. If the displayed reaction volume is the same as your reaction volume, go to step 6.
  9. If the displayed reaction volume differs from your reaction volume, enter the volume of your reactions (5-100 µl) in the Reaction Volume field.
  10. To clear an entry, press the CE key.
  11. Press F1-Start to start a run. When the heated cover reaches 1030C, the Run Time screen displays and the method you selected starts running.
  12. At the end of the run you can either remove the PCR tubes immediately or they will be held at 40C until you can remove them for storage in a refrigerator.
  13. To create new programs/methods refer to the Instruction Manual.

QUESTIONS/PROBLEMS: SEE DR. DRYDEN

 

TV/VCR

  1. Power on TV
  2. Set to Channel 3
  3. Power on VCR
  4. Set to Channel 2
  5. Insert tape
  6. Press play

QUESTIONS/PROBLEMS: SEE DR. McGRAIN

 

Video projectors, LED (Panasonic and Proxima)

(for Power Point Show, for programs on CD-ROM or diskette, for videos)

  1. Turn on power strip (piggybacked to second strip)
  2. Turn on computer
  3. Remove lens cap
  4. Turn on projector switch
  5. Turn on projector power button (on top)
  6. Press Input button until desired image appears
  7. Focus with buttons
  8. Zoom with buttons (*note: same buttons are on remote control)
  9. Use menu buttons to change parameters - color, vertical position etc.

Power Point Presentation:

  1. Go to Power Point and open up file
  2. Select slide show/video show
  3. Click mouse for next slide...
  4. To go back to a slide:
  5. Click on D in lower left of screen
  6. Go
  7. Slide navigator and select by clicking

CD-ROM Program or Program from Diskette:

*Be sure CD-ROM has been installed on computer.*

  1. Press Escape key on keyboard
  2. Click on CD-ROM bar icon at bottom (the one to the right of Quick Time)

Videos:

  1. Use input button to switch to VCR
  2. Follow same procedure

To shut down system:

  1. Exit everything
  2. Remove diskette and CD-ROM
  3. Turn off Projector by pressing Power Button (on top)

MUST WAIT UNTIL FAN GOES OFF TO PROCEED TO NEXT STEP OR YOU WILL BLOW OUT THE $400 BULB!! 

sound goes off and standby light changes from yellow to red.

  1. Switch Off power switch
  2. Turn off computer via Start (wait until noise is gone)
  3. Switch off Power Strip

QUESTIONS/PROBLEMS: SEE DR. McGRAIN.

 

Video Projectors, EPSON PowerLite 735c Multimedia Projectors

Stored in Room 111& 311A

  1. Connect the power cord to the back of the projector and plug the other end into a grounded outlet.
  2. Remove the lens cover.
  3. Connect the projector to the computer using the blue-capped cable
  4. Locate the remote control if necessary in the tray in the back of the projector.
  5. Turn on the power.
  6. Press the Comp/EasyMP button on the remote control until your computer image is displayed.  If using a laptop, you may need to press Fn and then press the function key that lets you display external monitor. This button may have a screen icon on it or be labeled CRT/LCD.  For Macintosh see instruction manual in case."
  7. To raise the image press the blue foot release buttons on each side of the projector and lift the front.  Release the buttons to lock the feet in position.  You can fine-tune the height by rotating the feet.
  8. Rotate the focus ring to sharpen the image.  Rotate the zoom ring to reduce or enlarge the image.
  9. When finished, turn off the projector first, by pressing the red Power button on the projector or remote control.  You should see a confirmation message on the computer screen.
  10. Press the Power button again.  The power light flashes orange for about a minute and a half as the projector cools down.  After the projector has cooled, the orange light stays on and the exhaust fan shuts off.
  11. Turn off and disconnect any equipment plugged into the projector.
  12. Reposition the feet to their most compact position if adjusted.
  13. Replace the lens cap.
  14. Unplug the projector.

QUESTIONS/PROBLEMS:  SEE DR. MARCH OR DR. LONGBRAKE

 

Water purification system, Nanopure

  1. Press "on/standby/off" button twice, or until you hear the machine come on.
  2. Check to make sure reading is in optimal range of 9.0-18.3 MW -cm (may have to wait until this range is reached- if you have to wait more than a minute, do not use the water and report the problem IMMEDIATELY to Dr. Lee).
  3. Take water from either outlet- it is preferable to use the front outlet, not the hose.
  4. Return to standby by pressing "on standby off" once- display will read "STBY."
  5. Leave in standby mode when not in use.

QUESTIONS/PROBLEMS: SEE DR. LEE.

 

Yost Smart Classroom Steps

  1. Touch screen anywhere
  2. Touch “Projector and Screen” button
  3. Press “On” – might have to do more than once!
  4. Wait until on
  5. Press “Desk Top Computer” button – might have to do more than once!
  6. Screen: use manual button on wall
  7. Light switch next to screen switch on wall is for front lights – turn off during PP presentations
  8. Volume controls: on screen, computer, and wall

On Keyboard:

  1. To log on, use username and password
  2. Go to your web space OR insert CD
  3. For Windows XP, go to “My Computer”
  4. Show end
  5. Shut down

Laser Pointer:  found on remote

Wireless Mouse:  underneath on charger

  1. Use to click through slides and use as a cursor

When finished:

  1. Press red “Shutdown” button to end
  2. Press “Yes”
  3. Everything should now be off

Don’t shut down system until all talks are finished – takes approximately 3 minutes to warm up projectors.

QUESTIONS/PROBLEMS:  SEE DR. LEE


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